• Biomarker research
  • Molecular exploration

Gene expression Facility at INT - GeneXprINT

Molecular biology equipment and bioinformatics approaches

The GeneXprINT facility combines bioinformatics and molecular biology expertise for gene expression analysis in neuroscience. It is dedicated to the implementation of general molecular biology technologies, with a specialization in quantitative PCR approaches for transcriptomic analysis (complex samples and single cells). GeneXprINT also offers a genotyping service (mouse model) and training/consulting in molecular biology and bioinformatics.

The GeneXprINT facility provides the INT research teams with :

i) a fully-equipped « molecular biology » technical platform, organized and structured in accordance with the necessary GLP and HS requirements (sectorization, dedicated workbenches and equipment, forward motion). This technical platform enables to perform, in a controlled environment : nucleic  acid extraction, qualitative and quantitative nucleic acid analysis, PCR, quantitative real-time PCR (qPCR), digital PCR (dPCR), RNAseq library preparation, post-PCR manipulations, molecular cloning and plasmid preparation.

ii) a fully-equipped « patch-transcriptomics » technical platform, organized and structured in accordance with GLP and HS requirements for molecular biology. This specific organization enables electrophysiology experiments to be carried out in a controlled environment, ensuring the necessary conditions for downstream transcriptomic experiments using PCR, qPCR and RNAseq methods on single cells.

iii) a genotyping service (mouse models). Genotyping is carried out using a standardized PCR protocol, optimized for the use of hair follicles. This non-invasive sampling method is in line with an approach designed to improve animal welfare, and does not require any specific authorization. The reporting of results is part of a quality approach that ensures traceability of information between the animal house, the genotyping department and the research teams.

 iv) Theoretical and technical training, support and advice for bioinformatics analyses: sequence analysis, PCR and qPCR assay design.

Techniques

  • Genotyping
  • PCR real time PCR bulk and single cell
  • molecular quantitation

Équipements

  • Bioanalyzer
  • Blocs PCR
  • Fast prep
  • Fast QPCR QS7 (96 wells or gene expression array card)
  • Nanodrop 2000
  • Qubit

Training

You are looking for a platform to train one or more staff on a particular technique or equipment?

The GeneXprINT facility can be opened up to training personnel from outside INT, although the current scope of training is essentially INT staff.

Users can be trained in :

Good Laboratory Practice, HS in molecular biology

PCR: principle, primer design, optimization and implementation

qPCR and dPCR: principle, primers and probe design, data analysis (PCR prerequisites)
Training/transfer of genotyping skills

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