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Glial compartment involvement in the regulation of food intake and the development of obesity

The team works on the regulation of feeding behavior and associated energetic disorders (obesity, anorexia, cachexia) by characterizing the non-neuronal and neuronal mechanisms operating at the level of the bulbar and hypothalamic structures.

Our team projects are based on general physiology approaches and functional exploration (eating behavior, calorimetry, telemetry, forced feeding, stereotactic surgery, electrophysiology). The team has mouse models with energy imbalance, either induced (diet, inflammation) or genetic (KO models). Cell biology techniques (cell culture, Western blotting) and molecular techniques (qPCR) complete our analysis potential.

The project covered by the internship proposal is to determine the contribution of the glial compartment in the hypothalamus and brain stem in the regulation of food intake and glucose homeostasis. In this context, we are particularly interested in a peptide expressed by glia in both structures and having a strong anorectic power. We will develop a multidisciplinary approach combining molecular, cellular and physiological approaches to characterize the involvement of this peptide in energy homeostasis and its effectiveness in the treatment of diet-induced obesity.

Keywords

Food intake, Obesity, Glia, Connexin 43, Hypothalamus, Brainstem.

Techniques

Biochemistry, immunostaining, histology, microscopy, animal surgery, pharmacology, animal behavior, electroencephalogram (EEG), indirect calorimetry, stereotactic surgery.

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