Escape the rodent trap: using iPS-cell derived neurons for pain research
On the 6th of September 2019, Neuroschool PhD Program invites Angelika Lampert (Uniklinik RWTH Aachen University, Institute of Physiology (Neurophysiology)) for a seminar on using iPS-cell derived neurons for pain research.
A major challenge in human pain research is the translational gap – findings from mice are not easily translated to humans. Therefore, we established a human-induced stem cell (iPS-cell) based approach to differentiate the nociceptors of patients with genetic pain syndromes. This system allows us to study the role of voltage-gated sodium channels, e.g. Nav1.7, in action potential firing. Using patch-clamp electrophysiology, we find that Nav1.7 is not active during subthreshold depolarizations, but that its activity defines the action potential threshold and contributes significantly to the action potential upstroke.
Ultimately, our goal is to identify personalized medicine for individual, treatment-resistant patients using iPSC derived human nociceptors. We report an example of in-vitro predicted individualized treatment success in a Caucasian patient suffering from severe small fiber neuropathy (SFN) with a genetic variant in Nav1.9. Using iPSC-derived patient nociceptors, we tested an FDA-approved compound, lacosamide, which abolished the disease-specific phenotype in-vitro. The patient responded promptly with significant pain relief. Thus, this stem-cell-based approach ended a history of 10-years of severe pain for the patient.
September 6th, 2019 – Amphi 12, Bâtiment B, Luminy campus
9:30 am – Discussion among students
10:30 am – Seminar (open to everyone) – Amphi 12, Batiment B, Luminy Campus.
11:30 am – Discussion with speaker for PhD students
Registration on AMeTICE
Summer school on motor disabilities – European School Marseille
Following its call for scientific events in neuroscience, NeuroSchool is funding the twentieth editi ...Learn more
Professional meeting: inspiring careers after a PhD in Neuroscience
On Friday, April 26th, 2019 on the Saint-Charles campus (Salle des Voûtes), come and meet Neuroscie ...Learn more