Publishing in high impact factor journals most often requires the combination of complementary techniques. Many researchers in the field on integrative neurosciences still do not dare to use techniques looking at the protein level although they would improve the interpretation of their data and enable them to better target the mechanisms.
Due to their central role in biological functions, proteins and protein interactions control the mechanisms leading to healthy and diseased states in organisms. Several tools and methods for protein analysis exist. The activities and structures of proteins can be examined in vitro, in vivo, and in silico. This course is meant for all PhD students in life science. If possible, an example in pathology will be the start point to understand the goal of the method. The technic will then be presented and explained with a special attention given on its advantages and inconvenient in order to be able to read a publication with a critical point of view.
DAY 1 – May 24th
9:30-10:15 Bruno Coutard (AFMB) – Molecular Biology/ Recombinant protein expression
10:30-11:15 Bruno Coutard (AFMB) – Biochemistry / protein purification
11:30-12:15 Stéphane Betzi (ISCB) – Protein structure and experimental procedures for structure Elucidation
12:30 -14:00 Lunch
14:00-14:45 Stéphane Betzi (ISCB) – Protein/protein interactions studies (biochemical and biophysical methods)
15:00-15:45 Stéphane Betzi (ISCB) – Identification of small molecules protein modulator and drug-design
DAY 2 – May 25th
9:30-10:15 Igor Medyna (INMED) – Basic technics: Principle & microscopy analysis (epifluorescence, confocal)
10:30-11:15 Igor Medyna (INMED) – Dynamic technics: FRAP & FRET
11:30-12:15 Avais Daulat (CRCM) – Affinity purification-Mass spectrometry
12:30 -14:00 Lunch
14:00-14:45 Marianne Guiral (BIP) – Electrophoresis (SDS-PAGE, 2D), Western blot
15:00-15:45 Marianne Guiral (BIP) – Blue native electrophoresis
Please register on Ametice before May 9th.