CRMBM - UMR 7339

Center for Magnetic Resonance in Biology and Medicine

Directrice : Monique BERNARD - Chef du service CEMEREM : Maxime GUYE

Faculté de Médecine
27 Boulevard Jean Moulin
13385 Marseille CEDEX 05
France

The The Center for Magnetic Resonance (MR) in Biology and Medicine (CRMBM) conducts translational research by developing and applying MR methods and instruments (RM) to explore the morphology, metabolism and physiology of human diseases and associated animal models (rodents). With the support of methodological and engineering teams, our research teams aim at (i) better characterizing healthy and pathological states of the central nervous, cardiovascular and musculoskeletal systems and (ii) defining new diagnostic and/or therapeutic strategies.

Research is conducted on state-of-the-art MR scanners for rodents at CRMBM (Horizontal 4.7T, vertical 11.75T, and horizontal 7T Bruker systems) and for humans at CEMEREM, the clinical site of CRMBM (Timone University Hospital), equipped with 3 clinical Siemens MR scanners (1.5T, 3T and the first 7T whole-body MR scanner in France).

Pictures from the CRMBM laboratory

Research teams

The CRMBM team, Professor Ranjeva’s team, located at the CEMEREM and working on the human central nervous system, is affiliated to the neuroscience master’s program and can thus train neuroscience master’s students.

INPAC (Imaging Normal and Pathological Central nervous system) (Jean-Philippe Ranjeva (Human CNS) & Angèle Viola (Animal CNS))

The CNS team aims at providing relevant innovative in vivo and non-invasive MRI parameters on patients and rodent models for the better understanding of the pathophysiology, structural abnormalities and dysfunction accompanying or causing some of the main neurological diseases such as multiple sclerosis, epilepsy, and spinal cord injury impacting public health, in close relationships with clinicians from the team and external collaborators specialized also in Alzheimer’s disease, schizophrenia and brain maturation.

MRI explorations are conducted on high field (1.5T, 3T) and ultra high field (7T) clinical MR scanners and on preclinical MR scanners (4.7T, 7T, 11.75T) using advanced MR methods (Diffusion MRI, fMRI, rs-fMRI, MRSI, X-nucleus MRI, QSM, ASL, MTR, …).

Members

Jean-Philippe Ranjeva, Angèle Viola, Virginie Callot, Guillaume Duhamel, Maxime Guye, Jean Pelletier, Bertrand Audoin, Nadine Girard, Sylviane Confort-Gouny, Wafaa Zaaroui, Adriana Perles-Barbacaru, Yann Le Fur, Olivier Girard, Arnaud Le Troter, Adil Maarouf, Audrey Rico, Aude-Marie Grapperon, Annie Verschuren, Patrick Viout, Lauriane Pini, Claire Coste, Isabelle Varlet, Françoise Reuter, Marie-Pierre Ranjeva, Aurélien Massire, Ben Ridley, Henitsoa Rasoandriananina, Samira M’Chinda, Viktor Carvahlo, Andreaa, Simon Levy, Emmanuelle Robinet, Pierre Lehman, Fanelly Pariollaud. Total : 9 HDRs.

Research axes

Characterization of the pathophysiology and clinical consequences of central nervous system pathologies using advanced MRI techniques and neuropsychological and clinical data collected from patients with MS, epilepsy, amyotrophic lateral sclerosis, Alzheimer’s disease, spinal cord injury, schizophrenia and Parkinson’s disease.

Techniques

  • Molecular biology (PCR…)
  •  Biochemistry (Western blot…)
  • Cell culture
  • Immunostaining, histology, flow cytometry
  • Microscopy (fluorescence, confocal, electronic…)
  • Pharmacology
  • Brain imaging and stimulation – Man (fMRI, TMS…)
  • Brain imaging – Animal
  • Electroencephalography (EEG)
  • Medical data analysis

Keywords

Advanced Magnetic Resonance Imaging, fMRI, Spectroscopic MRI, Brain, Spinal Cord, Multiple Sclerosis, Epilepsy, Alzheimer, Amyotrophic Lateral Sclerosis, Stroke, Parkinson, High Field and Ultra High Field clinical and preclinical MR scanners.

Computational neuroscience - Disorders of the nervous system - Excitability, synaptic transmission, network functions - Human cognition and behavior - Novel methods and technology development

Magnetic resonance of rodent models of brain pathologies (Angèle Viola)

The overall purpose of the research undertaken in our team is to investigate the pathophysiological mechanisms of selected brain pathologies on murine models developed in the lab and obtained via collaborations with external research units. We use a multimodal approach combining state-of-the art MRI/MRS methods with biochemistry and histology methods. In vivo MRI and MRS permit longitudinal studies of the same subject and offer versatile approaches for defining the structural, functional and metabolic alterations associated with CNS pathologies. These studies have a translational potential since they provide the opportunity of comparative investigations in humans and models. Our preclinical studies aim at identifying structural, functional and metabolic markers associated with pathogenesis that could translate into clinical studies, and be used to assess disease progression and evaluate new therapeutic strategies.

Our studies are intended to increase fundamental knowledge on disease pathogenesis by improving the characterization of :

  1. structural and microstructural alterations (anatomical MRI, Diffusion Tensor Imaging (DTI), relaxometry),
  2. inflammation dynamics (MRI with contrast agent injection, vascular permeability quantitative MRI),
  3. hemodynamic dysfunctions (MR angiography, perfusion MRI),
  4. metabolic dysfunctions (1H-MRS, 31P-MRS, metabolic imaging),
  5. metabolic profiles by ex vivo NMR for the study of relations between genotype, disease development and metabolic phenotype.

Members

Angèle Viola, Adriana-Teodora Perles-Barbacaru, Emilie Pecchi, Isabelle Varlet, Nathalie Cuge. Total : 1 HDR.

Research axes

  1. Characterization of murine models of human diseases (mainly models of multiple sclerosis, cerebral malaria, ischemia).
  2. Pre-clinical evaluation of new therapeutic strategies
  3. Development of MRI/MRS techniques, and image analysis methods in collaboration with physicists and image processing experts of our laboratory.

Techniques

  • Brain imaging – Animal
  • Molecular biology
  • Biochemistry
  • Histology

Keywords

MRI, MRS, murine models, brain, neurometabolism, inflammation, myelin, models of multiple sclerosis, experimental cerebral malaria, infectious diseases

This team is not affiliated to the Neuroscience Master’s

Animal cognition and behavior - Disorders of the nervous system
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